UFS Baseline 2013 KA32 Results

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Contents

Goal

The goal of the UFS Baseline experiment was to establish the operational capability to simultaneously perform the same algal growth experiment using identical equipment, media, strain, experimental protocol, and analytical techniques across the 6 ATP3 testbed sites. A common algal species (Nannochloropsis oceanica KA32) was grown at all sites. The UFS Baseline Experiment was intended as a first live run-through using the full volume and capabilities of the 6 identical ATP3 ponds to build pond operator familiarity with the equipment and procedures in an effort to eliminate operator error from the upcoming UFS Production runs.

Experimental Protocol

Here is the experimental protocol used to conduct these runs.

ATP3 Baseline UFS Protocol.pdf

Pond Operational Data

Click [here] for the complete operational data for each pond at each site. These data include the measured pond depth (cm), pH, salinity, pond water temperature (C), nitrogen concentration (ppm as N), phosphorus concentration (ppm as P), N:P ratio (molar), the sample ID and tracking ID of a physical sample (if taken), the algae concentration (g/L) as dry weight and ash-free dry weight (AFDW), the ash content of the algal biomass (%), and the optical density at 750nm (OD750). Not all data are available for all sites and ponds.

Instrumentation Data

Click [here] for the complete and daily-averaged instrumentation data for each pond at each site. These data include the pH, oxidation-reduction potention (ORP, mv), pond water temperature (C), pond conductivity (ms.cm), dissolved oxygen concentration (DO, mg/L), dissolved oxygen saturation (%), salinity (g/L), and photosynthetically active radiation (PAR, umol/m2/s). The PAR sensor was connected to only one of the ponds. Not all data are available for all sites and ponds.

Weather Data

Click [here] for the complete and daily-averaged weather data for each site. These data include air temperature (C), relative humidity (%RH), Global Light Intensity (W/m2/s), daily precipitation (cm), wind speed (km/hr), and wind direction (degrees). Not all data are available for all sites.

Summary Harvest Data

The UFS Baseline experiment was an initial startup, equipment, and operator trial run thus no harvest data was collected. This trial run had the potential to be highly variable due to unfamiliarity with equipment and operations, operator error, equipment failure, or other unforeseen events that commonly occur upon startup of complex systems. In summary, during the UFS Baseline experiment, few of these events occurred successfully meeting the goals of the ATP3 UFS Baseline experiment.

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